Nuclease assay

{
  "id": "MLC-T-CHM-008125",
  "term": "Nuclease assay",
  "field": "Chemistry",
  "definition": "A method for detecting or measuring a specific target nucleotide sequence by using a nuclease to cut nucleic acid and reading the result. In the 5' nuclease assay of real-time PCR, the polymerase's exonuclease activity cleaves a probe bound to the target and frees a fluorophore, while in nuclease protection assays, a probe annealed to the target survives digestion of unbound nucleic acid.",
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1150 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/nuclease-assay/"
}