Reversed-phase chromatography

{
  "id": "MLC-T-CHM-010321",
  "term": "Reversed-phase chromatography",
  "field": "Chemistry",
  "definition": "A chromatographic technique where the stationary phase is nonpolar and the mobile phase is polar, typically an aqueous solvent mixture. Separation occurs based on hydrophobic interactions between the analytes and the stationary phase, with more nonpolar compounds being retained longer. This is the most widely used mode of high-performance liquid chromatography.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "chromatography"
  ],
  "references": [
    "Orange Book, 2nd ed., p. 93 (https://media.iupac.org/publications/analytical_compendium/)",
    "PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 826 (https://doi.org/10.1351/pac199365040819)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/reversed-phase-chromatography/"
}