Hydrophobic-interaction chromatography

{
  "id": "MLC-T-CHM-005489",
  "term": "Hydrophobic-interaction chromatography",
  "field": "Chemistry",
  "definition": "A chromatographic method for separating biomolecules, primarily proteins, based on differences in their surface hydrophobicity. It employs a stationary phase with hydrophobic ligands and an aqueous mobile phase with a high salt concentration, which promotes hydrophobic interactions. Elution is achieved by decreasing the salt concentration, thereby reducing the hydrophobic interaction between the analyte and the stationary phase.",
  "subject": "Analytical Chemistry",
  "references": [
    "PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 187 (https://doi.org/10.1515/pac-2017-0111)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/hydrophobic-interaction-chromatography/"
}