Secondary screen

{
  "id": "MLC-T-CHM-013533",
  "term": "Secondary screen",
  "field": "Chemistry",
  "definition": "A set of assays performed on compounds identified as 'hits' from a primary high-throughput screening campaign. These tests are designed to confirm the activity of the initial hits, eliminate false positives, and provide more detailed information about their biological or chemical properties. A secondary screen often includes dose-response curves to determine the potency (EC50 or IC50) of the active compounds.",
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1154 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/secondary-screen/"
}