Total mobile phase volume

{
  "id": "MLC-T-CHM-012140",
  "term": "Total mobile phase volume",
  "field": "Chemistry",
  "definition": "The retention volume, in size-exclusion chromatography, of a sample component whose molecules are small enough to enter every pore of the packing particles, so that it samples all of the mobile phase in the column. It equals the total mobile phase time multiplied by the column flow rate, Vt = tt x Fc. It is the sum of the interparticle and intraparticle volumes, and larger molecules elute earlier, between V0 and Vt.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "retention volume",
    "total mobile phase time"
  ],
  "references": [
    "PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 212 (https://doi.org/10.1515/pac-2017-0111)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/total-mobile-phase-volume/"
}