Z factor

{
  "id": "MLC-T-CHM-012789",
  "term": "Z factor",
  "field": "Chemistry",
  "definitions": [
    "A dimensionless statistic for judging how well a screening assay performs in the presence of test compounds, calculated as 1 minus three times the sum of the standard deviations of the library sample signal and the control signal, divided by the absolute difference of their means. Values from 0.5 up to 1 are taken to mean an excellent assay. It differs from the Z′ factor, which is calculated from positive and negative controls alone, without test compounds.",
    "A dimensionless statistic that describes the quality of a screening assay itself, without any test compounds, calculated as 1 minus 3 times the summed standard deviations of the high control and the low control, divided by the absolute gap between their means. A value from 0.5 up to 1 indicates an excellent assay, and a value below 0 means the control signal bands overlap. It differs from the Z factor, which uses the library sample signal in place of the high control."
  ],
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1156 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/z-factor/"
}