Edman degradation

{
  "id": "MLC-T-CHM-003576",
  "term": "Edman degradation",
  "field": "Chemistry",
  "definition": "A sequential method for determining the amino acid sequence of a peptide or protein from its N-terminus. Phenyl isothiocyanate reacts with the N-terminal amino acid under mildly alkaline conditions, forming a phenylthiocarbamoyl derivative. This derivative is then cleaved under acidic conditions, releasing the N-terminal amino acid as a phenylthiohydantoin (PTH) derivative, which can be identified chromatographically.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "N-terminal analysis"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1155 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/edman-degradation/"
}