Hyperchromic effect

{
  "id": "MLC-T-CHM-005509",
  "term": "Hyperchromic effect",
  "field": "Chemistry",
  "definition": "An increase in the absorption of ultraviolet light by a substance, such as DNA, due to changes in its molecular structure. This effect is observed when double-stranded DNA denatures into single strands, as the unstacked bases absorb more light. The hyperchromic effect is a key indicator of DNA denaturation and melting temperature.",
  "subject": "Organic and Biomolecular Chemistry",
  "see_also": [
    "auxochrome",
    "hypochromic effect"
  ],
  "references": [
    "PAC, 1996, 68, 2223. 'Glossary of terms used in photochemistry (IUPAC Recommendations 1996)' on page 2247 (https://doi.org/10.1351/pac199668122223)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/hyperchromic-effect/"
}