Hypochromic effect

{
  "id": "MLC-T-CHM-013176",
  "term": "Hypochromic effect",
  "field": "Chemistry",
  "definition": "A decrease in the molar absorption coefficient of a substance, resulting in a lower intensity of light absorption at a specific wavelength. This phenomenon is observed in nucleic acids when individual polynucleotide strands associate to form a double helix, as the stacking of base pairs restricts the resonance of the aromatic rings. The opposite phenomenon, an increase in absorbance upon denaturation of the double helix, is known as the hyperchromic effect.",
  "subject": "Organic and Biomolecular Chemistry",
  "see_also": [
    "hyperchromic effect"
  ],
  "references": [
    "PAC, 1996, 68, 2223. 'Glossary of terms used in photochemistry (IUPAC Recommendations 1996)' on page 2247 (https://doi.org/10.1351/pac199668122223)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/hypochromic-effect/"
}