Lineweaver-Burk plot

{
  "id": "MLC-T-CHM-013237",
  "term": "Lineweaver-Burk plot",
  "field": "Chemistry",
  "definition": "A graphical representation of enzyme kinetics, created by plotting the reciprocal of the reaction velocity against the reciprocal of the substrate concentration. This double-reciprocal plot linearizes the Michaelis-Menten equation, yielding a straight line. The y-intercept of the line corresponds to the reciprocal of the maximum velocity (1/Vmax), while the x-intercept represents the negative reciprocal of the Michaelis constant (-1/Km).",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "Michaelis constant",
    "Michaelis-Menten kinetics"
  ],
  "references": [
    "PAC, 1994, 66, 2587. 'Glossary of bioanalytical nomenclature - Part 1: General terminology, body fluids, enzymology, immunology (IUPAC Recommendations 1994)' on page 2593 (https://doi.org/10.1351/pac199466122587)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/lineweaver-burk-plot/"
}