Protein purification

Term · Chemistry · MLC-T-CHM-009583

A series of processes intended to isolate a single type of protein from a complex mixture, such as cells, tissues, or body fluids. The methods exploit differences in protein properties like size, charge, and binding affinity, using techniques including chromatography and electrophoresis. A successful purification protocol results in an enrichment of the target protein, with purity often assessed by SDS-PAGE, where a single band indicates high homogeneity.

Table 1. Record
Identifier	MLC-T-CHM-009583
Field	Chemistry
Subject	Analytical Chemistry
References	PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1161 (https://doi.org/10.1515/pac-2016-1120)
See also	Immunoprecipitation

Record as JSON

{
  "id": "MLC-T-CHM-009583",
  "term": "Protein purification",
  "field": "Chemistry",
  "definition": "A series of processes intended to isolate a single type of protein from a complex mixture, such as cells, tissues, or body fluids. The methods exploit differences in protein properties like size, charge, and binding affinity, using techniques including chromatography and electrophoresis. A successful purification protocol results in an enrichment of the target protein, with purity often assessed by SDS-PAGE, where a single band indicates high homogeneity.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "immunoprecipitation"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1161 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/protein-purification/"
}

Record 10,181 of 13,676 in Chemistry terminology (MLC-0109). Request the full dataset.