Radial immunodiffusion

{
  "id": "MLC-T-CHM-009805",
  "term": "Radial immunodiffusion",
  "field": "Chemistry",
  "definition": "A quantitative immunoassay in which antigen placed in a well diffuses into agar containing a specific antibody and forms a ring of precipitate where the two reach equivalence. In the endpoint (Mancini) method the square of the ring diameter is proportional to antigen concentration, so readings from standards give a calibration line for unknowns. Serum immunoglobulins and complement components were routinely measured this way before nephelometry largely replaced it.",
  "synonyms": [
    "Mancini immunodiffusion"
  ],
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1238 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/radial-immunodiffusion/"
}