Ouchterlony technique

{
  "id": "MLC-T-CHM-008367",
  "term": "Ouchterlony technique",
  "field": "Chemistry",
  "definition": "A double immunodiffusion method used to detect and identify antibodies and antigens by observing precipitin lines formed in an agar gel. Antigens and antibodies are placed in separate wells and allowed to diffuse towards each other, forming visible lines of precipitation where they meet at optimal concentrations. This technique can distinguish between reactions of identity, partial identity, and non-identity.",
  "synonyms": [
    "double diffusion",
    "Ouchterlony double diffusion assay"
  ],
  "subject": "Chemistry and Human Health",
  "see_also": [
    "precipitating antibody"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1223 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/ouchterlony-technique/"
}