Size-exclusion chromatography

Term · Chemistry · MLC-T-CHM-010921

A chromatographic technique used to separate macromolecules, such as polymers, based primarily on their hydrodynamic volume or size in solution. The separation occurs as molecules pass through a porous stationary phase, with larger molecules eluting first because they are excluded from smaller pores. This method is widely used to determine the molecular weight distribution of polymers.

Table 1. Record
Identifier	MLC-T-CHM-010921
Field	Chemistry
Subject	Polymer
References	Purple Book, 1st ed., p. 69 (http://old.iupac.org/publications/books/author/metanomski.html)

Record as JSON

{
  "id": "MLC-T-CHM-010921",
  "term": "Size-exclusion chromatography",
  "field": "Chemistry",
  "definition": "A chromatographic technique used to separate macromolecules, such as polymers, based primarily on their hydrodynamic volume or size in solution. The separation occurs as molecules pass through a porous stationary phase, with larger molecules eluting first because they are excluded from smaller pores. This method is widely used to determine the molecular weight distribution of polymers.",
  "subject": "Polymer",
  "references": [
    "Purple Book, 1st ed., p. 69 (http://old.iupac.org/publications/books/author/metanomski.html)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/size-exclusion-chromatography/"
}

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