Spot

{
  "id": "MLC-T-CHM-011245",
  "term": "Spot",
  "field": "Chemistry",
  "definition": "A zone of finite area on a chromatogram, typically circular or elliptical, where a separated component of a mixture is located. In planar chromatography techniques like paper or thin-layer chromatography, spots become visible after development, either because the components are naturally colored or through the use of a visualization agent. The position and intensity of a spot are used for qualitative and quantitative analysis of the substance, respectively.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "thin-layer chromatography"
  ],
  "references": [
    "Orange Book, 2nd ed., p. 96 (https://media.iupac.org/publications/analytical_compendium/)",
    "PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 834 (https://doi.org/10.1351/pac199365040819)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/spot/"
}