Gradient elution

{
  "id": "MLC-T-CHM-004978",
  "term": "Gradient elution",
  "field": "Chemistry",
  "definition": "A procedure in liquid chromatography where the composition of the mobile phase is deliberately changed during the separation process. The change in solvent strength or polarity is programmed to run over a specific time, altering the retention of analytes as they travel through the column. This technique contrasts with isocratic elution, where the mobile phase composition remains constant throughout the entire run.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "mobile phase"
  ],
  "references": [
    "Orange Book, 2nd ed., p. 93 (https://media.iupac.org/publications/analytical_compendium/)",
    "PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 826 (https://doi.org/10.1351/pac199365040819)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/gradient-elution/"
}