TdT-dependent dUTP-biotin nick end labeling assay

{
  "id": "MLC-T-CHM-011839",
  "term": "TdT-dependent dUTP-biotin nick end labeling assay",
  "field": "Chemistry",
  "definition": "A biochemical method used to detect DNA fragmentation, a hallmark of apoptosis, by labeling the free 3'-hydroxyl termini of DNA breaks. Terminal deoxynucleotidyl transferase (TdT) adds biotinylated deoxyuridine triphosphate (dUTP) to these ends, which are then detected using streptavidin conjugated to a fluorochrome or enzyme. This assay is widely employed in cell biology and toxicology to quantify apoptotic cells.",
  "synonyms": [
    "TUNEL assay"
  ],
  "subject": "Chemistry and Human Health",
  "see_also": [
    "terminal deoxynucleotidyl transferase"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1250 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/tdt-dependent-dutp-biotin-nick-end-labeling-assay/"
}