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Tunel test

Term · Chemistry · MLC-T-CHM-012357

A biochemical assay used to detect deoxyribonucleic acid (DNA) fragmentation, a hallmark of apoptosis or programmed cell death. This method relies on the enzyme terminal deoxynucleotidyl transferase (TdT) to label the free 3'-hydroxyl termini of DNA strand breaks with modified nucleotides. The labeled DNA fragments can then be visualized and quantified using fluorescence microscopy or flow cytometry.

Table 1. Record
IdentifierMLC-T-CHM-012357
FieldChemistry
SubjectAnalytical Chemistry
ReferencesPAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1186 (https://doi.org/10.1515/pac-2016-1120)
See alsoDeoxyribonucleic acid fragmentation; Strand break
Record as JSON
{
  "id": "MLC-T-CHM-012357",
  "term": "Tunel test",
  "field": "Chemistry",
  "definition": "A biochemical assay used to detect deoxyribonucleic acid (DNA) fragmentation, a hallmark of apoptosis or programmed cell death. This method relies on the enzyme terminal deoxynucleotidyl transferase (TdT) to label the free 3'-hydroxyl termini of DNA strand breaks with modified nucleotides. The labeled DNA fragments can then be visualized and quantified using fluorescence microscopy or flow cytometry.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "deoxyribonucleic acid fragmentation",
    "strand break"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1186 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/tunel-test/"
}

Record 13,174 of 13,676 in Chemistry terminology (MLC-0109). Request the full dataset.