Total internal reflection fluorescence

{
  "id": "MLC-T-CHM-012134",
  "term": "Total internal reflection fluorescence",
  "field": "Chemistry",
  "definition": "A microscopy technique that selectively illuminates and excites fluorophores within a very thin region (typically 100-200 nanometers) immediately adjacent to the interface between two media with different refractive indices. An evanescent wave, generated by total internal reflection of an incident laser beam, penetrates only a short distance into the sample, minimizing background fluorescence from the bulk solution. This method is particularly effective for studying surface phenomena and single-molecule dynamics.",
  "subject": "Chemistry and Human Health",
  "references": [
    "PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1155 (https://doi.org/10.1351/PAC-REC-09-05-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/total-internal-reflection-fluorescence/"
}