Blocking

{
  "id": "MLC-T-CHM-001382",
  "term": "Blocking",
  "field": "Chemistry",
  "definition": "The process of reducing non-specific binding of analytes or reagents to surfaces in bioanalytical assays. This is achieved by treating the surface with a blocking agent, such as a protein or detergent, that occupies sites where non-specific interactions could occur. Common blocking agents include bovine serum albumin (BSA) or casein.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "biomacromolecule",
    "tissue"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1137 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/blocking/"
}