Double-wavelength spectroscopy

{
  "id": "MLC-T-CHM-003422",
  "term": "Double-wavelength spectroscopy",
  "field": "Chemistry",
  "definition": "A spectroscopic method that measures absorbance or transmittance at two different wavelengths simultaneously or in rapid succession. One wavelength is chosen for maximum absorption by the analyte, while the other serves as a reference to correct for background interference or matrix effects. This technique is frequently employed in clinical chemistry for the determination of specific analytes in complex biological samples.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "absorbance",
    "scattering",
    "spectroscopy",
    "wavelength"
  ],
  "references": [
    "PAC, 1988, 60, 1449. 'Nomenclature, symbols, units and their usage in spectrochemical analysis - VII. Molecular absorption spectroscopy, ultraviolet and visible (UV/VIS) (Recommendations 1988)' on page 1455 (https://doi.org/10.1351/pac198860091449)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/double-wavelength-spectroscopy/"
}