Difference absorption spectroscopy

{
  "id": "MLC-T-CHM-003161",
  "term": "Difference absorption spectroscopy",
  "field": "Chemistry",
  "definition": "A spectroscopic technique that measures the difference in absorbance between two samples or between a sample and a reference. This method is particularly useful for detecting small changes in a system, such as conformational changes in biomolecules or reaction intermediates. The resulting spectrum highlights only the components that differ between the two measured states.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "absorber",
    "matched cells",
    "precision",
    "spectroscopy"
  ],
  "references": [
    "PAC, 1988, 60, 1449. 'Nomenclature, symbols, units and their usage in spectrochemical analysis - VII. Molecular absorption spectroscopy, ultraviolet and visible (UV/VIS) (Recommendations 1988)' on page 1455 (https://doi.org/10.1351/pac198860091449)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/difference-absorption-spectroscopy/"
}