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Fluorescein isothiocyanate

Term · Chemistry · MLC-T-CHM-004515

A reactive derivative of fluorescein, commonly used as a fluorescent label in biological and chemical applications. It contains an isothiocyanate group that reacts with primary amines, forming a stable thiourea linkage. This compound emits green fluorescence when excited by blue light, with an excitation maximum around 495 nm and an emission maximum around 520 nm.

Table 1. Record
IdentifierMLC-T-CHM-004515
FieldChemistry
SubjectChemistry and Human Health
ReferencesPAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1166 (https://doi.org/10.1351/PAC-REC-11-06-03)
See alsoImmunofluorescence
Record as JSON
{
  "id": "MLC-T-CHM-004515",
  "term": "Fluorescein isothiocyanate",
  "field": "Chemistry",
  "definition": "A reactive derivative of fluorescein, commonly used as a fluorescent label in biological and chemical applications. It contains an isothiocyanate group that reacts with primary amines, forming a stable thiourea linkage. This compound emits green fluorescence when excited by blue light, with an excitation maximum around 495 nm and an emission maximum around 520 nm.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "immunofluorescence"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1166 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/fluorescein-isothiocyanate/"
}

Record 4,791 of 13,676 in Chemistry terminology (MLC-0109). Request the full dataset.