Gram stain

{
  "id": "MLC-T-CHM-004992",
  "term": "Gram stain",
  "field": "Chemistry",
  "definition": "A differential staining technique used to classify bacteria into two large groups: Gram-positive and Gram-negative, based on the chemical and physical properties of their cell walls. The procedure involves crystal violet, iodine, decolorizer, and safranin. Gram-positive bacteria retain the crystal violet stain and appear purple, while Gram-negative bacteria do not and appear red or pink.",
  "subject": "Chemistry and the Environment",
  "see_also": [
    "gram",
    "lipopolysaccharides",
    "membrane",
    "peptidoglycan"
  ],
  "references": [
    "PAC, 1992, 64, 143. 'Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992)' on page 155 (https://doi.org/10.1351/pac199264010143)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/gram-stain/"
}