Pull-down assay

{
  "id": "MLC-T-CHM-009664",
  "term": "Pull-down assay",
  "field": "Chemistry",
  "definition": "A biochemical technique used to isolate and identify proteins that interact with a specific bait protein or other molecule, such as DNA or RNA. The bait molecule is immobilized on a solid support, and cellular lysates are passed over it, allowing interacting partners to bind. The bound complexes are then eluted and analyzed, often by gel electrophoresis and mass spectrometry.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "immunoprecipitation",
    "protein-protein interaction"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1185 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/pull-down-assay/"
}