Insert

{
  "id": "MLC-T-CHM-005896",
  "term": "Insert",
  "field": "Chemistry",
  "definition": "A segment of deoxyribonucleic acid (DNA) that has been introduced into a vector, such as a plasmid or viral genome, for cloning or expression. This DNA fragment typically contains a gene or regulatory sequence of interest. The insert is ligated into the vector using restriction enzymes and DNA ligase, forming recombinant DNA.",
  "subject": "Chemistry and the Environment",
  "see_also": [
    "deoxyribonucleic acids",
    "insertion",
    "sequence",
    "vector"
  ],
  "references": [
    "PAC, 1992, 64, 143. 'Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992)' on page 158 (https://doi.org/10.1351/pac199264010143)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/insert/"
}