Tetramer staining

{
  "id": "MLC-T-CHM-011906",
  "term": "Tetramer staining",
  "field": "Chemistry",
  "definition": "A laboratory technique used to identify and quantify antigen-specific T lymphocytes by employing soluble major histocompatibility complex (MHC) molecules complexed with specific peptides. These MHC-peptide complexes are multimerized into tetramers and conjugated to a fluorochrome. The stained cells are then detected and analyzed using flow cytometry.",
  "subject": "Chemistry and Human Health",
  "see_also": [
    "T lymphocyte",
    "clonal expansion",
    "major histocompatibility complex class I molecule",
    "peptide tetramer"
  ],
  "references": [
    "PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1252 (https://doi.org/10.1351/PAC-REC-11-06-03)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/tetramer-staining/"
}