Two hybrid system

{
  "id": "MLC-T-CHM-012371",
  "term": "Two hybrid system",
  "field": "Chemistry",
  "definition": "A molecular biology technique used to detect protein-protein interactions in vivo, typically within a yeast cell. It involves fusing two proteins of interest to separate domains of a transcription factor, where interaction between the proteins reconstitutes the transcription factor's activity. This reconstitution then activates a reporter gene, providing a measurable signal of the interaction.",
  "subject": "Analytical Chemistry",
  "see_also": [
    "protein-protein interaction"
  ],
  "references": [
    "PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1186 (https://doi.org/10.1515/pac-2016-1120)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/two-hybrid-system/"
}