Photoaffinity labelling

{
  "id": "MLC-T-CHM-008803",
  "term": "Photoaffinity labelling",
  "field": "Chemistry",
  "definition": "A technique used to identify and characterize biomolecule binding sites by forming a covalent bond between a ligand and its receptor upon photolysis. A photoreactive group, such as a carbene or nitrene precursor, is incorporated into a ligand. Irradiation with ultraviolet light activates the photoreactive group, leading to irreversible attachment to nearby amino acid residues within the binding site.",
  "subject": "Organic and Biomolecular Chemistry",
  "see_also": [
    "binding site",
    "carbenes",
    "label",
    "nitrenes",
    "proteins",
    "receptor"
  ],
  "references": [
    "PAC, 2007, 79, 293. 'Glossary of terms used in photochemistry, 3rd edition (IUPAC Recommendations 2006)' on page 383 (https://doi.org/10.1351/pac200779030293)"
  ],
  "url": "https://mlchart.com/terminology/chemistry/photoaffinity-labelling/"
}